Automated Synthetic Cell-Based Screening for Designed Proteins with Emergent Functions

Screening protein libraries for complex functions remains a major challenge. Here the authors present an automated microfluidic platform for protein library screening that facilitated the redesign of the bacterial cell division protein FtsZ and discovery of protein modulators of FtsZ. The platform utilizes a combination of microfluidic devices and machine learning algorithms to rapidly screen large libraries of proteins for specific functions. This approach enables researchers to identify novel protein-protein interactions and design proteins with emergent functions. The authors demonstrate the effectiveness of this platform by redesigning the FtsZ protein and discovering protein modulators that enhance its function. These findings have significant implications for the development of new therapeutic strategies and the understanding of protein function. The automated microfluidic platform presented in this study represents a significant advancement in protein screening technology and has the potential to accelerate the discovery of novel proteins with emergent functions. However, it is essential to note that this technology is intended for research-use only and should not be used for therapeutic or diagnostic purposes without proper regulatory approval and guidance. Further research is needed to fully understand the potential applications and limitations of this technology.