Deciphering the evolutionary origin of the enantioselectivity of short-chain dehydrogenases from plants toward 1-borneol
Enzyme engineering has enabled optimization of enzymes for biotechnological processes, however, less is known about how natural evolution creates new functionalities. Here, the authors investigate the evolutionary emergence of enantioselectivity in plant borneol dehydrogenases using ancestral sequence reconstruction, structural analysis, site-directed mutagenesis and computational simulations.
Enzyme engineering has enabled optimization of enzymes for biotechnological processes, however, less is known about how natural evolution creates new functionalities. Here, the authors investigate the evolutionary emergence of enantioselectivity in plant borneol dehydrogenases using ancestral sequence reconstruction, structural analysis, site-directed mutagenesis and computational simulations.