Native yeast kinetochore structures identify an essential inner kinetochore interaction
Accurate kinetochore assembly at centromeres is essential for proper chromosome segregation. Here, the authors purified and visualized native yeast inner kinetochore complexes, revealing that Ndc10 trimerization bends ce
Kinetochores must accurately assemble on centromeres for faithful chromosome segregation. The authors' purification protocol allows for the study of native yeast inner kinetochore subcomplexes, providing new insights into the structural dynamics of this complex. Cryoelectron microscopy reveals that Ndc10 trimerization is essential for kinetochore assembly and chromosome segregation. The findings suggest a crucial role for centromeric DNA bending in stabilizing the centromeric nucleosome and inner kinetochore. This study contributes to our understanding of yeast kinetochore assembly, highlighting the importance of structural studies in elucidating the mechanisms underlying this process.